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Figure 3 | BMC Genomics

Figure 3

From: Statistics of protein-DNA binding and the total number of binding sites for a transcription factor in the mammalian genome

Figure 3

Suboptimal design of the ChIP-qPCR experiment. Statistics of BEs in Esrrb TF ChIP-seq data is following to skewed distribution. Difference in the frequency distributions of BEs for peaks used in qPCR and in random samples chosen from Esrrb TF ChIP-seq library at peak values >11 available for this dataset. In Chen et al [15], to determine the specificity, the peak height critical threshold were determined by 3-fold enriched qPCR signal/noise criteria.

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