Figure 6

Multiple occurrence of c-Myc E-boxes in promoter region around transcription start site (TSS) of c-Myc target genes identified in ChIP-seq experiment. A: WEE 1 homolog 1(Wee1). The high-avidity (height peak = 76) c-Myc binding sites (pointed by blue arrow) in strong promoter region of Wee1 gene is supported with two canonical E-box CACGTG. The binding locus and E-boxes are overlapped with CpG Island which might be bound by c-Myc. B: Nucleoplasmin 3 (Npm3). The moderate-avidity (height peak = 10) as in the previous case the ChIP-seq c-Myc binding locus is located in the first intron promoter region. The locus is supported with five E-boxes: one canonical and three non-canonical E-boxes in first intron and another canonical E-box in second exon. In addition, this binding region and the E-boxes are located in CpG Island. C: FK506 binding protein 5 (Fkbp5). Two relatively low avidity c-Myc binding sites identified in ChIP-seq experiment confirmed with E-boxes. First ChIP-seq loci in upstream gene region has relatively low avidity biding site (height peak = 7) supported with canonical E-box CACGTG. The second ChIP-seq loci located in first intron and it is also relatively low avidity peak (height peak = 7) which is supported with two non-canonical E-boxes CACGCG and two non-canonical E-boxes CGCGAG. The last locus overlaps with CpG Island which suggests that this locus might be functional.