Figure 2

TNFAIP1/POLDIP2 complex sense antisense architecture mapped onto the genome (UCSC genomic browser). A - TNFAIP1/POLDIP SAGP (red arrows) and TMEM97/IFT20 SAGP (two next closest blue arrows on the left) with seven other genes included in the analysis (blue arrows). B - TNFAIP1/POLDIP2 complex cis-sense antisense architecture (red box) with different tracks. Small green solid boxes represent CpG islands, and green transparent boxes represent regions of enrichment of potential miRNA regulatory target sites. ChIP-Seq tracks represent regions of DNA binding by STAT1 (human cervical cancer HeLaS3 cell line [42]), ChIP-PET-defined histone trimethylations H3K4me3 and H3K27me3 (promyelocytic leukemia cell line HL60 [40]) and ChIP-seq-defined RNA polymerase II binding (breast cancer cell line MCF7 [44]). Black arrows at the bottom indicate the direct evidence of transcription activation of the TNFAIP1/POLDIP2 CSAGA in the breast cancer cell line. The GIS ChiP-PET track shows H3K4me3 and H3K27me3 regions mapped on the human genome (embryonic stem cell line hES3 [41]).