The flowchart of our interactome construction process 1. the LR score of each method is calculated first, we next combined the results LR of gene neighbor, gene fusion, phylogenetic profile and SSBP together and validated the PPI pair obtained by these four methods with protein domain method. Only the PPI that is predicted by one of the above four methods and is supported by domain could be integrated in our interactome. 2. We integrated the result of the newly combined methods with the results of ortholog and gene coexpression. The final LR score of our PPI network is the product of the LR scores of the three above methods.