Figure 1

Scheme of a genetic AND gate regulated by transcription factors. Assembly of two different operator parts forms a device that functions as a genetic AND gate. This AND gate integrates inputs of two different inducers into a transcription output. The pale blue arrow represents a -35/-10 sequence. The green rectangle represents an operator part inserted into the upstream of the -35/-10 sequence. The orange rectangle represents an operator part inserted into the downstream of the -35/-10 sequence. The black arrows pointing the operators represent the environmental inputs. The blue ellipse represents a ribosome-binding site. The gray arrow represents a coding region of a protein whose transcription is turned on as an output.