Figure 1

Consistency of microarray results and Q-PCR confirmation of selected genes Normalized microarray data of 5 cDNA clones of Tsc22 (A), 4 clones of Col3a1 (B) and 4 clones of Fubp1 (C) were compared between samples s1 and s2 (corresponding to 2 of the 4 mouse ovarian samples assayed) across 6 microarray replicates each. Adjusted p-values and expression change (ec), defined as the difference between log₂ averages are shown. In D, the coordinates of scatter plot depicts the average of normalized microarray ratios (5-6 replicates) and the average of duplicate Q-PCR assays for the genes Spp1, Txnrd1, Anxa5, Fn1, C1s, Ctsl, and Mt1 in each of the 4 mouse strains. The internal control transcript was the 18S rRNA. Q-PCR data was converted to log₂ scale ratios as described in Methods. The squared correlation coefficient (R²) shown corresponds to the overall gene subset in the 4 strains (i.e. 28 data pairs). Raw Q-PCR results and individual microarray ratios are detailed in Additional file1, table 1.