Association of TF-responsiveness in ES cells with tissue-specificity. (A, B) Scatterplot of average tissue-specificity measured by the information of mRNA abundance in: GNF and NIA databases, respectively, versus TF-responsiveness (B
); each dot represents the average for 100 genes with similar TF-responsiveness. (C) Percent unique variance of tissue-specificity (information) explained by TF-responsiveness (B
), presence of CpG island (CpG), presence of TATA box, and interaction of the two later factors (CpG*TATA), based on multi-variate linear regression; "n/s" = non-significant, otherwise it is significant (p < 0.05). (D) Fraction of the top 1000 tissue-specific genes that fall into four categories based on the presence of a CpG island (CpG) and TATA box. (E) Comparison of TF-responsiveness in tissue-specific genes from the GNF database (same as D) and in other genes, all differences are significant (p < 0.05).