Functional annotation of the genes containing nsSNPs. A. Gene ontology (GO) terms enriched in the 100 genes containing the highest number of nsSNPs. Blue columns show the percentage of genes among these nsSNP-containing genes, and red columns show the percentage of genes within the whole genome. In this chart, the secondary level terms are used as GO terms. B. Identified nsSNPs reportedly associated with phenotypes in the other breed of cattle. Six SNP sites exactly matched mutations reported in previous studies to be associated with economically important traits [25, 26, 29, 30, 35]. "-" implies the absence of record in the dbSNP database. EBV: estimated breeding values; SCS: somatic cell score.