Figure 8

Rates of specific nt substitutions. Rates of the indicated nt substitutions at individual positions were tabulated separately for forward and reverse direction reads. The average rate of a given substitution per position bearing the indicated initial nt within 5C.C7 (A, D), OT-1 (B, E), and DO11.10 (C, F) sequence sets was calculated. The median (line), 25-75 percentile error rate (box), and range (whiskers) of this for the 27 sequencing reactions per TCR are plotted for phred unfiltered (A-C) or q = 30 (D-F) filtered sequences.