Figure 4

Activation of the CWI pathway in mutant ccw12 Δ. Mutant ccw12 Δ, slt2 Δ, gas1 Δ and BY4741 (isogenic wt) cells, were grown to mid-exponential phase, and cell extracts were analysed by Western blot using phospho-p44/p42 MAPK antibodies to detect dually phosphorylated Slt2p (mid and low panel, PP-Slt2p). Pfk1p was used as a loading control (upper panel, Pfk1p). PP-Slt2p and Pfk1p protein levels were quantified as described in Figure 1