Budding and formation of the mating projection are affected in ccw12 Δ cells. Wt (SEY6211) and ccw12 Δ mutant (MEY12B) strains were treated with the mating pheromone α-factor as detailed in Methods. At time zero 20 μg/ml of α-factor were added. Budding index (A) and shmoo formation (B) was monitored over the time. Mean values of four independent experiments are shown. At least 200 cells were analysed at the indicated times.