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Table 1 Genes showing synthetic interaction with CCW12

From: The genetic interaction network of CCW12, a Saccharomyces cerevisiae gene required for cell wall integrity during budding and formation of mating projections

ORF Gene Description of gene product SGA Fold change(a) Interaction in other cell wall networks(b) Published interaction(c)
     Array qRT-PCR   
   Cell wall synthesis and regulation      
YBL061C CHS4 Activator of Chs3p (chitin synthase III), recruits Chs3p to the bud neck via interaction with Bni4p ss    FKS1, SMI1, GAS1 -
YBR023C CHS3 Chitin synthase III ss 2.5 3.2 ± 0.7 FKS1, SMI1, GAS1 [27, 30]
YDR245W MNN10 Subunit of the Mannan polymerase II complex sl    CHS3, CHS4, CHS5, CHS6, CHS7, SMI1 -
YHR030C SLT2 MAP-kinase of the cell integrity pathway sl 2.2 1.7 ± 0.1 BNI4, CHS3, CHS4, CHS5, CHS6, CHS7, FKS1, GAS1, SMI1 [31]
YHR142W CHS7 Involved in chitin biosynthesis by regulating Chs3p export from the ER ss    FKS1, SMI1, GAS1 [27, 30, 31]
YJL095W BCK1 MAPKK-kinase of the cell integrity pathway sl 2.5 2.7 ± 0.5 BNI4, CHS1, CHS3, CHS4, CHS5, CHS7, GAS1, SMI1 [31]
YLR113W HOG1 Mitogen-activated protein kinase involved in osmoregulation ss     -
YLR330W CHS5 Involved in export of selected proteins, such as chitin synthase Chs3p, from the Golgi to the plasma membrane sl    FKS1, SMI1, GAS1 [27, 30]
YLR332W MID2 Sensor for the PKC1-SLT2 cell wall integrity pathway ss    FKS1, SMI1 [27, 29, 31]
   Cell polarity and vesicular transport      
YBL007C SLA1 Cortical actin patch assembly control protein, mutation affects endocytosis sl 2.7 2.7 ± 0.1 CHS3, CHS4, CHS5, CHS7, FKS1, SMI1 [31]
YBL047C EDE1 Cortical actin patch protein with a role in endocytosis ss 2.6 2.7 ± 0.5 BNI4, CHS3, CHS4, CHS5, CHS7, FKS1 [31]
YCR034W FEN1 Involved in fatty acids elongation ss    FKS1 -
YDR129C SAC6 Involved in the organization and maintenance of the actin cytoskeleton sl    CHS3, CHS4, CHS5, CHS7 -
YJL179W PFD1 Involved in the biogenesis of actin and of alpha- and gamma-tubulin sl 2.5 1.7 ± 0.4   -
YLR370C ARC18 Required for the motility and integrity of cortical actin patches sl    CHS1, CHS3, CHS4, CHS5, CHS6 -
YNR051C BRE5 Activator of Ubp3p that regulates COPII coat assembly ss    BNI4, CHS3, CHS4, FKS1, SMI1 [31]
YOR035C SHE4 Involved in cortical actin patch assembly and endocytosis ss    CHS3, CHS4, CHS7, FKS1 [31]
   Other genes      
YLR119W SRN2 Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome ss     -
YNL197C WHI3 Regulates the critical cell size required for passage through Start ss     -
YDR417C   Hypothetical protein, potential GPI anchor and secretion sequence ss     -
YGR078C PAC10 Polypeptide 3 of a Yeast Non-native Actin Binding Complex sl     -
  1. (a) Data correspond to the ratio of expression of ccw12 versus wt calculated as described in "Methods".
  2. (b) [30, 60]
  3. (c) Published Genetic Interactions with CCW12.