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Table 1 Genes showing synthetic interaction with CCW12

From: The genetic interaction network of CCW12, a Saccharomyces cerevisiae gene required for cell wall integrity during budding and formation of mating projections

ORF

Gene

Description of gene product

SGA

Fold change(a)

Interaction in other cell wall networks(b)

Published interaction(c)

    

Array

qRT-PCR

  
  

Cell wall synthesis and regulation

     

YBL061C

CHS4

Activator of Chs3p (chitin synthase III), recruits Chs3p to the bud neck via interaction with Bni4p

ss

  

FKS1, SMI1, GAS1

-

YBR023C

CHS3

Chitin synthase III

ss

2.5

3.2 ± 0.7

FKS1, SMI1, GAS1

[27, 30]

YDR245W

MNN10

Subunit of the Mannan polymerase II complex

sl

  

CHS3, CHS4, CHS5, CHS6, CHS7, SMI1

-

YHR030C

SLT2

MAP-kinase of the cell integrity pathway

sl

2.2

1.7 ± 0.1

BNI4, CHS3, CHS4, CHS5, CHS6, CHS7, FKS1, GAS1, SMI1

[31]

YHR142W

CHS7

Involved in chitin biosynthesis by regulating Chs3p export from the ER

ss

  

FKS1, SMI1, GAS1

[27, 30, 31]

YJL095W

BCK1

MAPKK-kinase of the cell integrity pathway

sl

2.5

2.7 ± 0.5

BNI4, CHS1, CHS3, CHS4, CHS5, CHS7, GAS1, SMI1

[31]

YLR113W

HOG1

Mitogen-activated protein kinase involved in osmoregulation

ss

   

-

YLR330W

CHS5

Involved in export of selected proteins, such as chitin synthase Chs3p, from the Golgi to the plasma membrane

sl

  

FKS1, SMI1, GAS1

[27, 30]

YLR332W

MID2

Sensor for the PKC1-SLT2 cell wall integrity pathway

ss

  

FKS1, SMI1

[27, 29, 31]

  

Cell polarity and vesicular transport

     

YBL007C

SLA1

Cortical actin patch assembly control protein, mutation affects endocytosis

sl

2.7

2.7 ± 0.1

CHS3, CHS4, CHS5, CHS7, FKS1, SMI1

[31]

YBL047C

EDE1

Cortical actin patch protein with a role in endocytosis

ss

2.6

2.7 ± 0.5

BNI4, CHS3, CHS4, CHS5, CHS7, FKS1

[31]

YCR034W

FEN1

Involved in fatty acids elongation

ss

  

FKS1

-

YDR129C

SAC6

Involved in the organization and maintenance of the actin cytoskeleton

sl

  

CHS3, CHS4, CHS5, CHS7

-

YJL179W

PFD1

Involved in the biogenesis of actin and of alpha- and gamma-tubulin

sl

2.5

1.7 ± 0.4

 

-

YLR370C

ARC18

Required for the motility and integrity of cortical actin patches

sl

  

CHS1, CHS3, CHS4, CHS5, CHS6

-

YNR051C

BRE5

Activator of Ubp3p that regulates COPII coat assembly

ss

  

BNI4, CHS3, CHS4, FKS1, SMI1

[31]

YOR035C

SHE4

Involved in cortical actin patch assembly and endocytosis

ss

  

CHS3, CHS4, CHS7, FKS1

[31]

  

Other genes

     

YLR119W

SRN2

Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome

ss

   

-

YNL197C

WHI3

Regulates the critical cell size required for passage through Start

ss

   

-

YDR417C

 

Hypothetical protein, potential GPI anchor and secretion sequence

ss

   

-

YGR078C

PAC10

Polypeptide 3 of a Yeast Non-native Actin Binding Complex

sl

   

-

  1. (a) Data correspond to the ratio of expression of ccw12 versus wt calculated as described in "Methods".
  2. (b) [30, 60]
  3. (c) Published Genetic Interactions with CCW12.
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BMC Genomics

ISSN: 1471-2164

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