Figure 3

Confirmation of SOX2 regulated genes and microRNAs. (A) Bar graph showing PCR confirmation of the array data of SOX2 gene expression. SOX2-KO: SOX2 knockdown; MOCK, mock known down with negative control siRNAs. Y-axis, relative expression after normalizing the MOCK control to value of 1; X-axis, gene symbols. (B). Bar graph showing PCR confirmation of the microRNA sequencing data. Y-axis, relative expression after normalizing the MOCK control to value of 1; X-axis, microRNA names. (C). Western blot analysis showing the SOX2 expression comparing microRNA 145 precursor mimics and a scrambled negative control. (D). A VENN diagram showing the overlaps of SOX2 targets in GBM and ES cells, and the genes that changed expression (2 fold cutoff) after SOX2 knockdown in GBM cells.