Figure 3From: The living microarray: a high-throughput platform for measuring transcription dynamics in single cellsGlobal expression changes. 293T cells were reverse-transfected with inducible constructs, each arrayed over 75 replicate spots, and fluorescent cells were imaged over 24 hours. Single-cell measurements at each spot were averaged for the Venus (orange) and ECFP (blue) channels and are shown over time for (A) p(GRE)x3-AdMLP-VC5 following induction with 1 × 10-7M dexamethasone, (B) pTetRE-VC5 following induction with 2.2 × 10-6M doxycycline, (C) p(RARE)x3-AdMLP-VC5 following induction with 1 × 10-6M all-trans retinoic acid and (D) the promoterless VC5 reporter. The mean of all replicate fields is drawn in red for Venus signal and black for ECFP signal.Back to article page