Figure 4
Single-cell measurements of promoter activity. Single-cell measurements are plotted at 0 (blue) and 24 hours (red) post-induction for (A) EF1α-VC5 and (B) p(GRE)x3-AdMLP-VC5. (C) Multi-dimensional view of inducing cells on a single spot. Spatial movement of cells from one field is shown where each cell is represented by a different color and the intensity of normalized Venus fluorescence is proportional to the size of the dot. (D-F) 293T cells transfected with p(GRE)x3-AdMLP-VC5 were tracked over 24 hours after induction with 1 × 10-7M dexamethasone. Fluorescence measurements for single cells having induced over 3 standard deviations above the initial mean are displayed for (D) ECFP and (E) Venus. (F) Single-cell expression profiles normalized for ECFP signal. Darker lines represent the distribution of normalized cell intensities at 1 and 15 hours following treatment, illustrating cellular heterogeneity in the transcriptional response.