Figure 1

Overview of genome resequencing and SNP allele determination. (A) 454 GS FLX sequencing technology was used for shotgun genome sequencing to fine-map crossovers and other structural variants in 2 clonal progeny lines of P. falciparum. (B) WGS reads of the progeny genomes as well as the WGS reads from the parent genomes [28] were mapped to the common reference sequence (strain 3D7, PlasmoDB 5.4 [27]). (C) High quality SNPs (see methods) captured in parent and progeny genomes were used for allele screening.