Figure 2
From: Applicability of RNA standards for evaluating RT-qPCR assays and platforms
Limit of detection of ERCC RNA standards. Incidence of failed PCR reactions on ABI 7900HT system or BioMark using cDNA or pre-amplified cDNA as the template. Mean percentage of failed PCR reactions ± SD are displayed based on data from 8 different RNA standards from 3 independent qPCR experiments across a range from 1 to 106 copies ERCC standard per ng total RNA.