Skip to main content

Table 2 Concentrations and ratios of ERCC RNA standards in simulated 'normal' and 'disease' panels

From: Applicability of RNA standards for evaluating RT-qPCR assays and platforms

ERCC standard

Copies/ng total RNA

Ratio B/A

 

Panel A

Panel B

 

13

1 × 105

1 × 105

1.0

25

1 × 102

1.5 × 102

1.5

42

1 × 104

5 × 103

0.5

51

5 × 100

1 × 102

20.0

81

1 × 102

1 × 102

1.0

84

1 × 102

5 × 102

5.0

95

1 × 103

1 × 103

1.0

99

8 × 103

1.2 × 104

1.5

113

1 × 101

1 × 101

1.0

171

1 × 101

1 × 102

10.0

  1. 10 ERCC RNA standards were spiked in a background of Universal Human Reference RNA at different concentrations and ratios in order to create simulated 'normal' and 'disease' panels, A and B.
Back to article page

BMC Genomics

ISSN: 1471-2164

Contact us