Table 7 Passive Cutaneous Anaphylaxis (PCA) assay in BALB/c mice. A blue wheal of a diameter of 3 mm or more, 30 min following challenge was considered as positive response and indicative of the presence of allergen-specific IgE. * p < 0.05
| Â | Treatment | Diameter (mm) | Positive Reaction | |
|---|---|---|---|---|
Groups | Sensitization | Allergen Challenge | Mean ± SEM | (n) Responder/Total |
|  | Naïve Serum | PNA | 0.9 ± 0.07 | 0/3 |
Naïve Control | Naïve Serum | OVA | 0.8 ± 0.15 | 0/3 |
|  | Naïve Serum | BLG | 1.0 ± 0.03 | 0/3 |
|  | PBS SC Serum | PNA | 1.1 ± 0.13 | 0/3 |
PBS Control | PBS SC Serum | OVA | 1.1 ± 0.21 | 0/3 |
|  | PBS SC Serum | BLG | 1.4 ± 0.26 | 0/3 |
Experimental Group 1 | PNA SC Serum | PNA | 5.1 ± 0.43* | 4/4 |
Experimental Group 2 | OVA SC Serum | OVA | 4.5 ± 0.74* | 3/4 |
Experimental Group 3 | BLG SC Serum | BLG | 4.1 ± 0.72* | 3/4 |