Figure 1

General architecture of TRAM software. The user is guided step-by-step through import and analysis of any gene expression profile dataset in text format. The gene identifiers of any type are converted in official gene symbols/gene names, followed by intra-sample as well as inter-sample normalization of gene expression values. The expression is mapped along each chromosome and graphically displayed on the basis of mean value for all genes included in each segment of arbitrary length. Over- and under-expressed regions are determined following statistical analysis.