Distinct colonization patterns and cDNA-AFLP transcriptome profiles in compatible and incompatible interactions between melon and different races of Fusarium oxysporum f. sp. melonis

Sestili, Sara; Polverari, Annalisa; Luongo, Laura; Ferrarini, Alberto; Scotton, Michele; Hussain, Jamshaid; Delledonne, Massimo; Ficcadenti, Nadia; Belisario, Alessandra

doi:10.1186/1471-2164-12-122

Table 2 Selected list of Fusarium oxysporum f. sp. melonis (FOM) transcripts expressed in melon plants during infection.

From: Distinct colonization patterns and cDNA-AFLP transcriptome profiles in compatible and incompatible interactions between melon and different races of Fusarium oxysporum f. sp. melonis

ID	Bst-Mse	Length (bp)	Accessions	Annotation	Blast score	Category	ISPaVe1070				ISPaVe1018				ISPaVe1083
							2 dpi	4 dpi	8 dpi	21 dpi	2 dpi	4 dpi	8 dpi	21 dpi	2 dpi	4 dpi	8 dpi	21 dpi
TDFs expressed only *in planta*
P1425	TG-CG	277	FOXT_01463	hypothetical protein similar to actin binding protein	3E-76	cell component	0	0	0	0	0	0	0	2	0	0	0	2
P1443	TA-CG	190	FOXG_00066	dynamin-2	6E-76	cell component	0	0	0	0	0	0	0	1	0	0	0	1
P406	CC-TT	479	FOXT_05654	hypothetical protein similar to 1,4-beta-glucanase	7E-124	cell wall	0	0	0	0	0	0	0	2	0	0	0	1
P998	CT-TG	174	FOXT_00113	chitin synthase 4	3E-52	cell wall	0	0	0	0	0	0	0	1	0	0	0	1
P1261	TG-AT	209	AF080485	Fusarium oxysporum f. sp. lycopersici pectate lyase	2E-87	cell wall-degrading	0	0	0	0	0	0	0	1	0	0	0	2
P553	CT-GT	235	FOXG_09638	endo-1,4-beta-xylanase 2 precursor	7E-101	cell wall-degrading	0	0	0	1	0	0	0	0	0	0	0	0
P1362	TC-GC	377	FOXT_13415	endo-1,4-beta-xylanase	9E-145	cell wall-degrading	0	0	0	0	0	0	0	2	0	0	0	1
P32	TT-AA	148	FOXG_06061	squalene synthetase	1E-42	metabolism	0	0	0	0	0	0	0	1	0	0	0	1
P675	CG-TT	259	FOXT_08412	protein similar to phosphoserine phosphatase	1E-114	signal transduction	0	0	0	0	0	0	0	1	0	0	0	1
P1473	TG-AG	224	FOXT_00565	hypothetical protein similar to calnexin	4E-102	signal transduction	0	0	0	0	0	0	0	1	0	0	0	1
P842	CT-AA	382	FOXG_03102	hypothetical protein similar to MADS box protein	4E-131	signal transduction	0	0	1	1	0	1	1	2	0	1	1	2
P1331	TC-CG	165	FVET_00351	hypothetical protein similar to vps28 protein	3E-24	transport	0	1	0	0	0	0	0	1	0	0	0	1
P888	TC-TA	449	FOXT_11289	plasma membrane ATPase	1E-178	transport	0	0	0	0	0	0	0	1	0	0	0	1
P1014	TT-GA	140	FOXT_12267	hypothetical protein similar to hexose transporter	8E-25	transport	0	0	0	0	0	0	0	2	0	0	0	1
P1066	CC-GC	380	FOXT_11113	mitochondrial phosphate carrier protein	1E-177	transport	0	0	0	0	0	0	0	1	0	0	0	1
P1232	CA-AT	341	FOXT_10521	mitochondrial import inner membrane translocase	1E-63	transport	0	0	0	0	0	0	0	1	0	0	0	1
P819	TC-AC	359	FOXG_09125	hypothetical protein similar to avenacinase	6E-117	virulence	0	0	0	0	0	0	0	3	0	0	0	1
P1258	TG-AT	468	FOXG_09782	siderophore iron transporter mirB	0	virulence	0	0	0	0	0	0	0	1	0	0	0	1
P1482	TC-TC	187	FOXT_03354	hypothetical protein similar to Fum16p	3E-81	virulence	0	0	0	0	0	0	0	2	0	0	0	2
TDFs expressed both *in planta* and *in vitro*
P417	CC-TT	168	FOXT_11103	hypothetical protein similar to sterol esterase precursor	6E-57	lipid metabolism	0	0	0	0	0	0	0	2	0	0	0	1
P292	TT-CG	180	FOXT_00536	UTP-glucose-1-phosphate uridylyltransferase	1E-77	metabolism	0	0	0	0	0	0	0	2	0	0	2	2
P305	CG-CA	199	FOXG_09447	pyruvate dehydrogenase E1 component subunit alpha	2E-88	metabolism	0	0	0	0	0	0	0	2	0	0	0	1
P344	CG-CC	363	FOXG_11520	NAD-specific glutamate dehydrogenase	1E-103	metabolism	0	0	0	0	0	0	0	3	0	0	0	2
P1211	CA-GC	159	FOXG_10419	malate dehydrogenase, mitochondrial precursor	1,00E-67	metabolism	0	0	0	0	0	0	0	2	0	0	0	2
P1333	TC-CG	140	FOXT_11398	UDP-glucose 6-dehydrogenase	4,00E-58	metabolism	0	0	0	0	0	0	0	3	0	0	0	3
P1486	CC-CA	226	FOXT_07937	glutathione reductase	1E-89	metabolism	0	0	0	0	0	0	0	1	0	0	0	1
P1280	TG-TC	358	FOXT_00266	hypothetical protein similar to multiubiquitin	1E-60	protein metabolism	0	0	0	0	0	0	0	1	0	0	0	1
P201	TA-TA	443	FOXG_03710	hypothetical protein similar to proteasome regulatory	0	protein metabolism	0	0	0	0	0	0	0	1	0	0	0	1
P1398	TA-GG	286	FOXT_00231	proteasome component C5	2E-139	protein metabolism	0	0	0	0	0	0	0	1	0	0	0	1
P304	CG-CA	215	FOXT_10713	hypothetical protein similar to Ca2+ ATPase	4E-68	signal transduction	0	0	0	0	0	0	0	1	0	0	0	1
P960	CT-TT	187	FOXG_05517	cAMP-dependent protein kinase regulatory subunit	6E-79	signal transduction	0	0	0	0	0	0	0	1	0	0	0	1
P469	CT-CA	294	FOXT_12260	peroxidase/catalase 2	7E-148	stress response	0	0	0	0	0	0	0	2	0	0	0	2
P1134	CG-GG	352	FOXT_01866	sorting nexin-41	0	transport	0	0	0	0	0	0	0	1	0	0	0	1
P464	CC-TG	136	FOXT_09879	hypothetical protein similar to neutral amino acid permease	8E-40	transport	0	0	0	0	0	0	0	2	0	0	0	1
P611	CT-GC	284	FOXG_09522	hypothetical protein similar to metalloreductase	1E-139	transport	0	0	0	0	0	0	0	3	0	0	0	3
P1293	TC-CA	396	FVET_08954	hypothetical protein similar to vacuolar sorting protein 35	0	transport	0	0	0	0	0	0	0	1	0	0	0	1
P365	CG-CG	426	FOXT_12915	arginase	0	virulence	0	0	0	0	0	0	0	1	0	0	0	1
P610	CT-GC	291	FOXT_05960	hypothetical protein similar to peroxisomal PEX11	1E-136	virulence	0	0	0	0	0	0	0	2	0	0	0	1

Selected FOM transcripts identified in infected melon plants, either specifically in planta, or both in planta and in vitro. Identification numbers (ID) correspond to progressive numbering of bands in the gels. The primer combination used to visualize each band (Bst-Mse column) and the corresponding length of the fragment are reported, along with the accession number in the database from which the sequence was retrieved, its annotation, the corresponding blast score and the functional category to which each transcript was assigned. In addition, the table reports the expression profile of each transcript, in each interaction and at each time point, estimated according to band intensity with a score from 1 to 3.

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ISSN: 1471-2164

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