Distinct colonization patterns and cDNA-AFLP transcriptome profiles in compatible and incompatible interactions between melon and different races of Fusarium oxysporum f. sp. melonis

Table 4 Real time RT-PCR expression analysis of selected Fusarium oxysporum f. sp. melonis (FOM) genes.

ID	Homology	E-value	Annotation	ΔCt	SD
P493	FOXT_12671	5E-152	Fusarium oxysporum f.sp lycopersici aspartate aminotransferase	3.43	0.11
P135	FOXT_08523	1E-48	Fusarium oxysporum f.sp lycopersici acyl-CoA desaturase	16.11	0.41
P1315	FOXG_05190	2E-79	Fusarium oxysporum f.sp lycopersici E3 ubiquitin-protein ligase	3.99	0.31
P819	FOXG_09125	2E-117	Fusarium oxysporum f.sp lycopersici hypothetical protein similar to avenacinase	10.24	0.04
P1403	FOXT_13204	7E-51	Fusarium oxysporum f.sp lycopersici predicted protein	7.06	0.77

Five Fusarium oxysporum f. sp. melonis transcripts, expressed in planta during infection according to cDNA-AFLP experiments, were selected for validation by Real time RT-PCR. Data are shown as the difference between the Ct of the gene analyzed and the Ct of melon actin (AY859055), used as a normalizer.

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