Figure 1

Bacterial growth and tissue response within the first 8 h post-infection. Proximal tubule of rat kidney infected by UPEC. A-C. Imaging by two-photon microscopy of infected kidney 3 h (A), 5 h (B), and 8 h (C) post-infection. Bacteria are shown in green, blood flow is labelled with red dextran, injected tubules are visualized by endocytosed blue dextran. Scale bar 30 μm. D. Relative expression of gfp+ by bacteria quantified by qRT-PCR. Measurements are normalized to the 3 h time point. ND - not detected. Error bars show SD, n = 3. E-G. Gene expression 3 h (E), 5 h (F), and 8 h (G) post-infection quantified by microarray. Each point represents one array probe, n = 3. Probes marked in red have adjusted P- values of <0.59 (E), <0.15 (F), and <0.05 (G).