Figure 2

Clustering of differentially expressed host genes into functionally defined categories. GO annotations of differentially expressed genes identified at 8 h post-infection were analyzed and visualized in a directed acyclic graph using the BINGO plugin in Cytoscape. Categories are shown as circles, the diameter corresponding to the amount of found genes in a category. The adjusted P-value for overrepresentation of differentially expressed genes was calculated for each category and color coded, white circles have no overrepresentation. Circles marked GO represent the root node of gene ontology categories and contain all found genes. A. Genes retrieved with the criteria adjusted P-value <0.05 and differential expression ≥2. The numbering corresponds to the numbers in B. B. Genes retrieved with the criterion adjusted P-value <0.5. Numbering represents GO categories, see supporting information S4. The left half of the graph includes all numbered categories in Figure 2A, the right half comprises categories involved in biological regulation.