Example of a corrected gene start site. The alignment shows orthologous sequences from five Burkholderia genomes. Dashes indicate identical sequence relative to B. thailandensis. Bold text shows the Prodigal-predicted gene start sites. The xenovorans start site (bold) adds an 8 amino acid leader sequence to the encoded protein. Underlined text indicates the revised start site for B. xenovorans, imposed to achieve consistency.