Tissue expression profiles of ZNF280B/ZNF280BY and ZNF280A/ZNF280AY. Both ZNF280BY and ZNF280AY were proved to be Y-specific genes via the male-specific PCR (lane marked with ♂). ZNF280BY is expressed predominantly in the testes and slightly in liver, adrenal gland and lymph node while the autosomal ZNF280B is expressed specifically in the testis. The ZNF280AY is expressed predominantly in the spleen and low in testis and brain, while the expression of the ZNF280A was not detected among the eight tissues studied. The β-actin gene was used as positive control. Te, testis; Li, liver; Ki, kidney; Sp, spleen; Br, brain (cerebrum); Ad, adrenal gland; Mu, muscle; Ly, lymph node; Ov, ovary; ♂, bovine male genomic DNA control; ♀, bovine female genomic DNA control; -, water; M, 1 kb DNA ladder.