From: A proteomics approach to decipher the molecular nature of planarian stem cells
Samples: | Whole planarian extracts, dissociated cell extracts, dissociated cell and sub-fractionated extracts. |
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Extraction Buffers: | SDS, urea/thiourea. |
Processing Sample: | (Precipitation procedure) Amersham 2D clean up kit, acetone, TCA-acetone. |
Isoelectric Focusing (1st Dimension): | (Immobiline Dry strip gels 24 cm) Linear pH 4-7, Linear pH 7-11, Non-linear pH 3-11. |
Other Modifications: | Trypsin inhibitors, general protease inhibitors, sonication. |