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Table 1 Variables taken into account for the establishment of the planarian proteomic protocol using 2D gels.

From: A proteomics approach to decipher the molecular nature of planarian stem cells

Samples:

Whole planarian extracts,

dissociated cell extracts,

dissociated cell and sub-fractionated extracts.

Extraction Buffers:

SDS,

urea/thiourea.

Processing Sample:

(Precipitation procedure)

Amersham 2D clean up kit,

acetone,

TCA-acetone.

Isoelectric Focusing

(1st Dimension):

(Immobiline Dry strip gels 24 cm)

Linear pH 4-7,

Linear pH 7-11,

Non-linear pH 3-11.

Other Modifications:

Trypsin inhibitors,

general protease inhibitors,

sonication.

  1. All the different variables affecting protein sample production and 2D gel electrophoresis are listed on this table.