Table 1 Primer sequences and amplicon length
From: Genome wide analysis of the bovine mucin genes and their gastrointestinal transcription profile
Gene | Forward Sequence | Reverse Sequence | ALa (bp) |
|---|---|---|---|
GAPDHb | GGGTCATCATCTCTGCACCT | GGTCATAAGTCCCTCCACGA | 176 |
GAPDH | ACCCAGAAGACTGTGGATGG | CAACAGACACGTTGGGAGTG | 178 |
ACTB | GACATCCGCAAGGACCTCTA | ACATCTGCTGGAAGGTGGAC | 205 |
MUC1 | CACTGCTGCCAGCCATATTA | TCAAACCCCAAATGCTTCTC | 212 |
MUC2 | CATGTGGAACCAGGAGGACT | ATGTTCCCAACCTCGACAGG | 153 |
MUC3A | CCGTCACAAGTACATCTAACACAG | ACATATTCGAGGCGTTAGCA | 150 |
MUC4 | GGAGAGTGCAGAGTCCTTGG | AGCAGCAAAGCCAATGAAGT | 323 |
MUC5AC | CAGACCCTCCACCTTCTTCA | GGTCCTCGAAGCTGTTCTTG | 263 |
MUC5B | TCTACCTGACCGTGGAGACC | GTTGATGATGCTGCACTGCT | 299 |
MUC6-1 | CAGCAAGGACAAAATCGTGA | CTCTGGTCTGGCCTCTGAAC | 218 |
MUC6-2 | CACGGCCTCCTGTCTTCTAC | AGAAGATGGACTGGCTCTCA | 190 |
MUC7 | TCCTGCTCCTAAGGCTACCA | GTGGAGGGGAGTGGTACTGA | 155 |
MUC12 | TACAGGGGCAACAACTTTCC | CGTCTCATCGTAGCACAGGA | 234 |
MUC13 | ACCTGGGACAGAGACACCAC | GATTGTCGGGGTAGGAGACA | 262 |
MUC15 | CTGCCTTGGAACTCATCCAT | CACAGACGTGGTGTTTGGTC | 183 |
MUC16 | TTCAGAAACAGCAGCATTGG | GTGTAGTGGTCCAGCCGAGT | 200 |
MUC19 | TTCTGGAGGGGCTGAATATG | CTCTCGTCCACCAAAAGAGC | 204 |
MUC20 | GGACATCACTGCTCTGACGA | GGGCTATTGTCCAGGTCTCA | 239 |
MUC21 | CAACCACAGGAGGCTCTGAT | CAAAGAATCCCACGACCACT | 173 |
BSM | GGAGACATATGGACTGCCAAT | CACAGTATGCAATTTCACAGCA | 194 |