Figure 9From: Gene expression analyses of immune responses in Atlantic salmon during early stages of infection by salmon louse (Lepeophtheirus salmonis) revealed bi-phasic responses coinciding with the copepod-chalimus transitionSet-up of the experiment. Atlantic salmon was challenged with L. salmonis at the copepod stage. Skin, spleen, and head kidney were sampled from challenged and control fish at 1, 3, 5 dpi (corresponding to the copepod stage); 10 and 15 dpi (chalimus stage). Boxes a, b, and c respectively indicate approximate positions on the fish where tissues (head kidney, skin, and spleen) were removed. A total of forty samples of spleen and skin form infected salmon (4 individuals from the 5 time points) were used for microarray analyses. Test samples were labeled with Cy5 and hybridized to pooled control samples labeled with Cy3 from the same time-points. Competitive hybridization to the arrays was followed by washing, scanning, image analysis, and data analysis. Selected genes were analyzed with RT-qPCR.Back to article page