Table 1 Primer information for genes selected for confirmation by qPCR.
Gene | GenBank Accession # | Forward primer (5'→3') | Reverse primer (5'→3') | Amplicon length, bp | Tm,°C |
|---|---|---|---|---|---|
Activin IIB Receptor | NM_204317 | GAGGAGCGCATTGCTCAGAT | GACAAGGCAGTCCGAGGTAGTG | 60 | 83 |
Beta parvin | XM_416459 | CCAGTCCATACAACTCGCCTTT | CTGTGGTAATACAGCTCACTGGATCT | 71 | 77 |
BTB domain containing 11 | XM_001234770 | TCTCAAGATGCTGATTTCTGTTTGT | GTATCGACACATCACTAGAGGAAAGATC | 74 | 75 |
Calmodulin 1 | NM_001110364 | GGCTGGATACACTTGGTGCAT | GGATCTGATGACCCAGGAAGTT | 68 | 80 |
Calreticulin | AY393845 | CGCCCTGACAACACCTATGA | CTCCAGGCTCCCCGATTC | 63 | 82 |
Caspase 3 | NM_204725 | GGAACACGCCAGGAAACTTG | TCTGCCACTCTGCGATTTACA | 63 | 78 |
Collagen, type VI, alpha 1 (COL6A1) | NM_205107 | TTCCATTGGTGCTCTTGCTATG | TTTGGGATGATGGCGATACC | 79 | 78 |
Death-associated Protein (DAP) | NM_001031003 | TGGGCAGCTCTACTCTATGTGTTC | CAAGAGCAGTTGTTTCTAACTCAAAAG | 79 | 78 |
Fibroblast growth factor 2 (FGF2) | NM_205433 | CAATCAAAGGCGTAAGTGCAAA | GCCAGCAATCTGCCATCCT | 61 | 80 |
Gelsolin | NM_204934 | GCTTACCCCCCTCGTCTTTT | TGAGTCAGATCTCCAGGGACTTC | 80 | 80 |
Glypican-1 | AY551002 | CCAGAGGTGGAGGTGGACAT | TCATGATCTTCAGCTGCATGATT | 73 | 80 |
Growth Arrest-Specific 2 (GAS2) | XM_420902 | TGATCCTTTCTGTTCCTGACAAAG | GGGAGAGGAAAAGGCAACCT | 75 | 80 |
Hypoxanthine phosphoribosyltransferase 1 (HPRT) | NM_204848 | CACAAGAAGCAGCCAGTTACAGTATC | CCCAGATTCCTTTTTACAGCAATAA | 92 | 80 |
Matrix Gla protein (MGP) | NM_205044 | GCACAATGCGTGCTCTCATC | AGCGGCCATGACCAAGAC | 56 | 84 |
Met proto-oncogene | NM_205212 | CAACAATCTTCTCCACTTTCATTGG | ATAGGGAGCAACACACTTCACGTT | 83 | 77 |
Myostatin | AF019625 | CACCTGGTACACCAAGCAAATC | TTGGTGGGTGTGCAGCAA | 59 | 83 |
Nebulin-related anchoring protein (NRAP) | XM_002194458 | GGCGTACCTTCGGGAAATG | GCAGAATTTCCGAAGCATCAG | 122 | 82 |
Nestin | NM_205033 | GCCCCGCGACAATCG | TGTTACAAACCAAAGGGAAATGG | 71 | 83 |
5'-nucleotidase, cytosolic III (NT5C3) | NM_204436 | TGTGGAGTGGGAAAGTCATACTGTT | CCTGTTGGTAAGAGGTTGAGAAACT | 77 | 77 |
Plastin 3 | NM_001006431 | TGCTGAGGGCACAGCTACAC | CAACTGAACCAAGATGCATACAAAA | 67 | 78 |
Platelet-derived growth factor A (PDGFA) | AB031021 | TGCAACACCAGCAGTGTGAA | TTTGCCACCTTGACACTTCTGT | 65 | 81 |
Q3_Reed_1DPH_cDNA_08_B09_130 (Unknown) | EH286878 | CCCTGTGCTCATCCTATAAAGCA | CCACGATGCAGCAGGTGAT | 61 | 82 |
Spondin 2 | XM_420847 | CGTTTTGTAGTTCTTCACCCTGCTA | TCACATTCTGTTTCTTCTTCCAGACT | 71 | 77 |
Syndecan-4 | AY852251 | GCCAACAGCAGCATCTTTGA | CAACTGCTCCTCCTGCAATG | 64 | 79 |
Troponin C type 1 (slow) (TNNC1) | NM_205133 | AGATGGACAGCCCGAATCTCT | TGGGACCAAGGAGCTGATG | 60 | 82 |
Troponin I type 1 (skeletal, slow) (TNNI1) | XM_419242 | TCTGCCCTCTTTGCACCATT | GTTGTCCACGTGGTTCATCTCA | 58 | 82 |
Troponin T type 2 (cardiac)(TNNT2) | NM_205449 | GGCTCAGCCATCAGATGCA | CAGCAGAGCCCTGGCATAG | 54 | 85 |
Troponin T type 3 (skeletal, fast) (TNNT3) | NM_204922 | CCCGTGCCTCAGTGATAACTAAA | AGAAGAAAAGCAGCAGCAATAGC | 68 | 78 |
Versican | NM_204787 | CAGGATCATTTGTTTGCGGTTA | TCCTGATTCTTCCGCAAGTCA | 60 | 80 |