Figure 4
Fatty acid biosynthesis pathway reconstructed based on the de novo assembly and annotation of D. tertiolecta transcriptome. Identified enzymes are shown in boxes and include: ACC, acetyl-CoA carboxylase (EC: 6.4.1.2); MAT, malonyl-CoA ACP transacylase (EC: 2.3.1.39); KAS, beta-ketoacyl-ACP synthase (KAS I, EC: 2.3.1.41; KASII, EC: 2.3.1.179; KAS III, EC: 2.3.1.180); KAR, beta-ketoacyl-ACP reductase (EC: 1.1.1.100); HAD, beta-hydroxyacyl-ACP dehydrase (EC: 4.2.1.-); EAR, enoyl-ACP reductase (EC: 1.3.1.9); AAD, acyl-ACP desaturase (EC: 1.14.19.2); OAH, oleoyl-ACP hydrolase (EC: 3.1.2.14); FatA, Acyl-ACP thioesterase A (EC: 3.1.2.-); Δ12D, Δ12(ω6)-desaturase (EC: 1.4.19.6); Δ15D, and Δ15(ω3)-desaturase (EC: 1.4.19.-). The fatty acid biosynthesis pathway in D. tertiolecta produces saturated, C16:0 (palmitic acid) and C18:0 (stearic acid), and unsaturated fatty acids C18:1 (9) (oleic acid), C18:2 (9,12) (linoleic acid), and C18:3(9,12,15) (α-linolenic acid). Key enzymes are shown with an asterisk next to the boxes.