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Table 1 D. tertiolecta transcriptome sequencing and assembly summary

From: Transcriptome sequencing and annotation of the microalgae Dunaliella tertiolecta: Pathway description and gene discovery for production of next-generation biofuels

 

Sequences

Bases (Mbp)

Sequencing

  

   Raw sequencing reads

1,385,389

567.6

   Average read length

410 bp

 

Pre-assembly

  

   Trimmed

352,422

 

   Trashed

20,036

 

   Reads used in assembly

1,365,353

546.6

   Average read length

400 bp

 

1Assembly

  

Contigs

  

   Reads assembled as contigs

609,149

258

   Number of contigs

34,301

 

   Average length of contigs

377 bp

 

   Range of contigs length

86-4,258 bp

 

   Depth on contigs

31

 

Isotigs

  

   Number of isotigs

33,307

17.8

   Average length of isotigs

535 bp

 

   Range of isotigs length

101-4,941 bp

 

   Depth on isotigs

2.1

 

Singletons

376,482

70.1

Unique sequence

409,789

 
  1. 1While this manuscript was in the review process, Newbler v2.5 became publicly available. We reassembled the D. tertiolecta transcriptome using Newbler v2.5 and the outcomes of assembly in comparison with those obtain from v2.3 are presented as the Additional file 1. The differences observed between the outputs of two versions were not significant to impact the major or minor conclusions of this study, and are consistent with reports from Ewen-Campen et al. [77].