Transcriptome sequencing and annotation of the microalgae Dunaliella tertiolecta: Pathway description and gene discovery for production of next-generation biofuels

Table 5 Enzymes involved in TAG biosynthesis and catabolism identified by annotation of the D. tertiolecta transcriptome

Enzyme	Symbol	EC Number	Number of transcripts	¹%Sequence alignment with corresponding enzymes in model organisms (Accession #)
				C. reinhardtii	V. carteri
TAG biosynthesis
Glycerol kinase	GK	2.7.1.30	16	42 (A8IYH1)	NM
Glycerol-3-phosphate O-acyltransferase	GPAT	2.3.1.15	0	²NM	NM
1-Acyl-sn-glycerol-3-phosphate O-acyltransferase	AGPAT	2.3.1.51	2	NM	NM
Phosphatidate phosphatase	PP	3.1.3.4	1	NM	NM
Diacylglycerol O-acyltransferase	DGAT	2.3.1.20	2	NM	NM
Phospholipid:diacyglycerol acyltransferase	PDAT	2.3.1.158	1	NM	NM
TAG catabolism				NM	NM
Triacylglycerol lipase	TAGL	3.1.1.3	2	NM	NM

¹In cases where multiple transcripts have been aligned with the associated enzymes in the model organisms, average similarity is reported.
²NM denotes that the annotated transcripts did not match the sequence of corresponding enzyme in model organisms.

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