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Table 6 Enzymes involved in starch biosynthesis and metabolism identified by annotation of the D. tertiolecta transcriptome

From: Transcriptome sequencing and annotation of the microalgae Dunaliella tertiolecta: Pathway description and gene discovery for production of next-generation biofuels

Enzyme

Symbol

EC Number

Number of transcripts

1%Sequence alignment with corresponding enzymes in model organisms (Accession #)

    

C. reinhardtii

V. carteri

Starch biosynthesis

     

Phosphoglucomutase

PGM

5.4.2.2

10

2NM

NM

ADP-glucose pyrophosphorylase

AGPase

2.7.7.27

7

49 (EDP04344.1), 84 (EDP08701.1)

NM

Starch synthase

SS

2.4.1.21

17

92 (EDP00372.1)

NM

1,4-α-Glucan branching enzyme

BE

2.4.1.18

6

87 (EDP05581.1)

82 (EFJ46288.1)

Sucrose phosphorylase

SuPase

2.4.7.1

3

NM

NM

Hexokinase

HXK

2.7.1.1

6

NM

NM

Starch catabolism

     

α-Amylase

(α-AMY)

3.2.1.1

9

98 (EDP00963.1)

NM

oligo-1,6-Glucosidase

(O1, 6G)

3.2.1.10

1

NM

NM

Starch phosphorylase

(SPase)

2.4.1.1

10

43 (EDP02951.1), 78 (EDO98385.1)

NM

  1. 1In cases where multiple transcripts have been aligned with the associated enzymes in the model organisms, average similarity is reported.
  2. 2NM denotes that the annotated transcripts did not match the sequence of corresponding enzyme in model organisms.