Figure 2
From: Proteomic analysis of the marine diatom Thalassiosira pseudonana upon exposure to benzo(a)pyrene
An example of tandem mass spectrometry spectra that exhibited significant iTRAQ ratios between BaP-exposed and control conditions. (A) The fragmentation spectrum of AIGGVTDGSVR, a peptide fragment from predicted C-5 cytosine-specific DNA methylase (B8BVP1) is shown. The reporter ion signals appear in the low-mass region of the spectrum and are used to determine the relative amount of the protein in T. pseudonana exposed to BaP (iTRAQ 114, 116 and 121) when compared to control conditions (iTRAQ 113, 115 and 119) (inset). (B-D) Reporter ion signals for other peptide fragments of B8BVP1.