Figure 1
From: Comparative transcriptome profiling of amyloid precursor protein family members in the adult cortex
Overview over study design. (a) Overview of APP processing. APP is first cleaved by either α-secretase or β-secretase, thereby shedding soluble APPsα or APPsβ, respectively. The membrane-bound C-terminal fragments (CTFs) are then cleaved by γ-secretase: αCTF gives rise to p3 and AICD whereas βCTF is cleaved to Aβ and AICD. (b) In APPα/α knock-in mice, a stop codon was introduced behind the α-secretase cleavage site by homologous recombination into the endogenous APP locus. Note that no full length APP or any other fragment can be generated from the APPsα knockin locus. (c) Overview of genotypes used for microarray analysis. APLP2(R1)-/- had been backcrossed for one generation to C57BL/6 whereas WT, APP-/-, APPα/α, APLP2-/- had been backcrossed for six generations. Six pairwise comparisons were performed: WT versus APP-/-, WT versus APPα/α, WT versus APLP2-/-, APPα/α versus APP-/-, WT versus APLP2(R1)-/-, and APLP2-/- versus APLP2(R1)-/-. The arrow indicates reference and tested group of each comparison.