Experimental design. Twelve C57BL/6J male mice were co-housed as pairs in six cages from weaning until 10 weeks of age (A). Liver, heart, kidney, and adipose tissues were collected from each mouse and split into two samples per tissue per individual and processed separately to generate RNA (B). In the case of kidney, the samples consist of the entire left or right kidney, respectively. Other tissues were chopped into small pieces, which were separated before placing them into collection tubes.