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Figure 2 | BMC Genomics

Figure 2

From: Genome-wide analysis of the role of GlnR in Streptomyces venezuelae provides new insights into global nitrogen regulation in actinomycetes

Figure 2

Verification of detection, immunoprecipitation and target promoter enrichment using anti-Flag antibody and Flag-tagged GlnR. (A) Western blot showing the presence of GlnR-Flag protein at each time-point described in Figure 1A; (B) the presence of GlnR-Flag in immuno-precipitates (IP) from whole-cell-lysates (WCE) was verified by Western blotting; (C) The presence of the promoter region of the GlnR target glnII, and absence of the promoter of the non-GlnR-regulated hrdB in immunoprecipitated samples (IP) was confirmed by PCR; total DNA (TD) was used as a control template.

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BMC Genomics

ISSN: 1471-2164

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