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Table 1 The 20 genes showing the greatest fold induction upon nitrogen starvation, that are also repressed by ammonium

From: Genome-wide analysis of the role of GlnR in Streptomyces venezuelae provides new insights into global nitrogen regulation in actinomycetes

Gene ID

S. coelicolor homologue

Annotation

Fold Induction in WT at T30

Fold Repression in Wt at T45

Sven_5279

SCO5583

ammonium transporter amtB

76.4

15.0

Sven_5281

SCO5585

PII uridylyltransferase glnD

26.0

8.3

Sven_2720

SCO2958

putative transcriptional regulator

21.6

6.0

Sven_5280

SCO5584

nitrogen regulatory protein PII glnK

16.0

6.8

Sven_2606

SCO2816

conserved hypothetical protein

15.4

3.9

Sven_0745

SCO1118

putative integral membrane protein

13.6

9.9

Sven_4809

SCO5163

unknown

12.5

3.7

Sven_0933

-

putative cholesterol esterase

11.7

7.2

Sven_6300

SCO6803

putative acetyltransferase

9.8

2.9

Sven_4564

SCO4896

putative transport integral membrane protein

9.2

7.3

Sven_1595

SCO1963

putative integral membrane export protein

8.7

2.2

Sven_3456

SCO6809

putative integral membrane transport protein

7.9

6.2

Sven_1523

-

unknown

7.7

4.5

Sven_2474

SCO5348

putative excisionase

7.4

2.7

Sven_3334

SCO3564

putative Na+/H+ antiporter

7.3

4.6

Sven_0739

SCO1109

putative oxidoreductase

7.2

4.4

Sven_3030

SCO3185

putative Na+/H+ antiporter

7.1

4.1

Sven_0867

SCO1293

putative acetyltransferase

6.7

5.2

Sven_1874

SCO2210

glutamine synthetase II glnII

6.5

3.6

Sven_3001

SCO3167

putative TetR-family transcriptional regulator

6.4

7.5

  1. Full list of genes induced >2 are shown in Additional file 2. Fold induction is calculated as the ratio signal T30/signal T0. Fold repression is the ratio signal T30/signal T45. Values given are the average of three hybridisations using cDNA generated from three independent biological experiments. Annotations taken from StrepDB database (http://strepdb.streptomyces.org.uk)