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Table 1 Target regions used in this study and the number of validated SNPs

From: Detection and mapping of mtDNA SNPs in Atlantic salmon using high throughput DNA sequencing

Region

Region size including primer binding sites (bp)

Amplicon number

Amplicon size excluding primer binding sites (bp)

Number of SNPs

SNPs per base-1

Average number of reads supporting the SNPs per individual

Multiallelic SNPs

Transitions

Transversion

Indels

DLOOPB

423

1

381

21

0,055118

25.4 (±10.3)

1

11

8

2

ND1

1161

2

384

15

0,039063

28.0 (±16.3)

1

13

2

 
  

3

369

5

0,013550

28.2 (±12.3)

 

5

  
  

4

324

8

0,024691

13.9 (±5.8)

 

8

  

ND2

770

5

361

13

0,036011

34.1 (±11.7)

 

13

  
  

6

346

7

0,020231

29.1 (±17.1)

 

5

2

 

COXI

821

7

372

12

0,032258

21.2 (±11.6)

 

12

  
  

8

382

12

0,031414

40.9 (±14.2)

 

11

1

 

COXII

715

9

361

7

0,019391

48.0 (±19.3)

 

7

  
  

10

311

7

0,022508

17.3 (±7.6)

 

7

  

ATP6

414

11

375

13

0,034667

35.9 (±14.1)

 

11

2

 

ND3

403

12

357

10

0,028011

30.5 (±12.8)

 

9

1

 

ND4

1181

13

363

12

0,033058

27.6 (±11.6)

 

12

  
  

14

361

13

0,036011

26.7 (±12.5)

 

13

  
  

15

370

13

0,035135

19.3 (±9.8)

 

12

1

 

ND5

783

16

345

8

0,023188

21.3 (±8.4)

 

8

  
  

17

370

12

0,032432

20.5 (±8.5)

 

12

  

CYTB

1161

18

366

10

0,027322

35.9 (±14.6)

 

10

  
  

19

352

8

0,022727

14.5 (±6.2)

 

8

  
  

20

365

12

0,032877

25.6 (±9.9)

1

9

3

 

Totals

7832

 

7215

218

0,030215

27.3 (±11.7)

3

196

20

2

  1. The amplicon sequences without primers within each region do not overlap (see Table in Additional material 4). Accordingly, the total number of bp analyzed from the mitochondrion DNA was 7215.