Phylogenetic tree of 16S rDNA of novel Clostridium sp. and Framework sequences. A neighbor - joining analysis with Jukes-Cantor correction and bootstrap support was performed on the rrs sequences of novel Clostridium sp. - 84 (shown in red) (as these could not be segregated among the 15 Clostridium sp. known to occur at high frequency and rest of the Clostridium spp. known to occur with low frequency (Additional file 6: Figures S18 and S19) along with 56 of phylogenetic framework (Figure 1). Bootstrap values are given at nodes. Sequences marked by filled square are the ones considered as framework in the study whereas type strains are indicated by 'T' as superscript. Values in parentheses are accession numbers (RDP and NCBI) (http://rdp.cme.msu.edu/ and http://www.ncbi.nlm.nih.gov/).