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Table 5 Restriction enzymes with unique in silico digestion pattern of 16S rDNA of different Clostridium species

From: Analysis of the unexplored features of rrs (16S rDNA) of the Genus Clostridium

Clostridium species

Restriction Enzyme (RE)

 

AluI

BfaI

DpnII

HaeIII

SmaI

Tru9I

RsaI

C. baratii

-a

Ub

-

-

-

U

-

C. butyricum

-

-

U

-

-

-

-

C. chauvoei

U

U

U

U

-

-

-

C. colicanis

-

U

U

U

U

-

-

C. kluyveri

U

U

U

U

U

-

-

C. novyi

-

-

-

-

-

U

U

C. pasteurianum

U

U

-

-

-

-

-

C. perfringens

-

U

-

-

-

-

-

C. sardiniense

-

-

-

-

-

U

 

C. subterminale

U

-

U

-

-

-

-

C. tetani

-

-

U

U

-

-

-

C. acetobutylicum (CAcI)c

U

U

-

-

-

-

-

C. botulinum (CBoI)c

-

U

-

-

-

-

-

  1. aNo unique digestion pattern was detected
  2. bIt denotes unique in terms of fragment length (nucleotides), order and number
  3. cIn the cases of C. acetobutylicum, C. beijerinckii, C. botulinum and C. sporogenes, no unique pattern could be detected and have been discussed separately. The different groups are based on their phylogenetic relationship (Figure 1). It is further assumed here that C. beijerinckii is a part of population comprised of C. acetobutylicum and similarly C. sporogenes is a part of C. botulinum.
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BMC Genomics

ISSN: 1471-2164

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