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Figure 7 | BMC Genomics

Figure 7

From: A systematic analysis of the skeletal muscle miRNA transcriptome of chicken varieties with divergent skeletal muscle growth identifies novel miRNAs and differentially expressed miRNAs

Figure 7

miR-1 directly targets the chicken ACVR2B UTR. A. Schema of miR-1 binding site in chicken ACVR2B 3'-UTR sequence (seed sequence highlighted in red). Mutated ACVR2B 3'-UTR eliminates the seed binding site pointed by arrow. B. Expression of miR-1 and ACVR2B gene in skeletal muscle of broiler and layer chickens at embryonic day 18 were analyzed using real-time RT-PCR. C. Target validation using a luciferase reporter assay. 293T cells were co-transfected with miR-1 mimic or scramble double-strand small RNA and the reporter plasmid pGL3 or pGL3-ACVR2B-UTR or pGL3-mACVR2B-UTR, respectively.

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BMC Genomics

ISSN: 1471-2164

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