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Table 4 List of grain quality genes with similar expression patterns in both SBS and MPSS libraries and up-regulated over five fold in Cypress (in comparison to LaGrue and Nipponbare) and in Ilpumbyeo (in comparison to YR15965 and Nipponbare)

From: Deep sequencing reveals the complex and coordinated transcriptional regulation of genes related to grain quality in rice cultivars

Up-regulated in Cypress

Family

TIGR Gene ID

TIGR gene name

MPSS signature

SBS signature

MPSS Ratio PSC/PSL

MPSS Ratio PSC/PSN

SBS Ratio PSC01/PSL01

SBS Ratio PSC01/PLN02

 

Starch synthesis and degradation

Os04g08270

Limit dextrinase, putative, expressed or alpha-amylase

GATCAGATACTCCTCAC

GATCAAATCTGAACCCATCA

49

16.3

8

8

 

Starch degradation

Os09g29404

Alpha-amylase activity

GATCTCCTCCTTTTCCT

GATCTGGAAGCGCGCCATTG

25

25

5

5

 

Glutelin

Os02g15070

Glutelin type-B 7 precursor

GATCCATTGCACAAGAG

GATCCAGCCACAAACCAATG

22

11

8

8

 

Starch synthesis and degradation

Os06g51084

1,4-alpha-glucan branching enzyme, chloroplast precursor

GATCAAGCAATGAATGC

GATCAACCCATGCTCCACCC

24

24

19

19

 

Seed specific

Os03g58480

Seed specific protein Bn15D14A

GATCACATCGTCACAGC

GATCTAGAATCTCCAGAGGG

14

28

7

7

 

Starch degradation

Os02g32660

Expressed 1,4-alpha-glucan branching enzyme

GATCATGACTTTCAGCA

GATCACAGAAGACACACTTC

6

24

8

8

 

Aspartate biosynthesis and degradation II + asparagine biosynthesis I and degradation I

Os02g14110

Nitrogen compound metabolism

GATCTGTGAATTTGGCA

GATCCAGAGAGAGATGCTAA

66

66

8

8

 

Globulin

Os03g46100

Globulin-1 S allele precursor

GATCATCCGCGCGTCGG

GATCGTTTAGTTGGGAGTGG

20

20

8

8

 

Seed maturation

Os09g10620

seed maturation protein LEA 4

GATCGAGTTGAGTGTGT

GATCGACTTGTGTGAGTTGT

16

16

8

8

Up regulated in Ilpumbyeo

Family

TIGR Gene ID

TIGR gene name

MPSS signature

SBS signature

MPSS Ratio PSI/PSY

MPSS Ratio PSI/PSN

SBS Ratio PSI02/PSY02

SBS Ratio PSI02/PSN02

 

Methionine degradation I

Os01g22010

Methionine adenosyltransferase

GATCCCGACTTCACATG

GATCCTCGCGGCCGAAATGG

35

35

14

14

 

Isoleucine biosynthesis I

Os03g21080

Acetolactate synthase

GATCGGAGCCTAGTTGC

GATCCAGCACACATTCAAAA

5

5

10

10

 

Starch synthesis

Os06g12450

Soluble starch synthase 2-3, chloroplast precursor

GATCTGGAAGTGAAATA

GATCTGGAAGTGAAATATTT

12

12

20

20

 

Seed allergenic/lectin

Os07g11510

Seed allergenic protein RA5 precursor

GATCGCCTCGCACCTGC

GATCACTTTAGTCTTTATAG

15

15

24

24

 

Starch degradation

Os02g32660

Expressed 1,4-alpha-glucan branching enzyme

GATCAGTGTTTTAAGTT

GATCAAATTACATATTGCTG

24.5

49

56

56

 

Starch synthesis

Os06g04200

Granule-bound starch synthase 1, chloroplast precursor

GATCTTCCACAGCAACA

GATCTTGGCAAGTCAATTAA

6

6

14

14

 

Isoleucine degradation I

Os02g43720

Enoyl-coA hydratase

GATCGTCTTGAAGGTCT

GATCTACCTCCATGCCTTGA

6.2

25

15

15

 

Aspartate biosynthesis and degradation II + asparagine biosynthesis I and degradation I

Os01g55540

Aspartate transaminase

GATCAAGTGGCTTTCAT

GATCGGCAAATACTCCTTAA

34

34

32

32