Table 2 Contigs representing the enzymes and proteins involved in oil biosynthesis

1

Pyruvate kinase

2

2179, 2717

2013*, 1637

Conversion of phosphoenol pyruvate to pyruvate

2

Pyruvate dehydrogenase E1a subunit

2

2503, 3695

1707*, 1317*

Conversion of pyruvate to acetyl-CoA

3

Pyruvate dehydrogenase E1b subunit

2

1782, 4154

1965*, 1203

Conversion of pyruvate to acetyl-CoA

4

Pyruvate dehydrogenase E2 subunit

3

1049, 1574, 11093

2430*, 2115, 517

Conversion of pyruvate to acetyl-CoA

5

Pyruvate dehydrogenase E3 subunit

2

1908, 3744

1934*, 1299

Conversion of pyruvate to acetyl-CoA

6

Lipoate synthase

1

10908

529

Conversion of pyruvate to acetyl-CoA

7

Lipoyl transferase

1

6252

864

Conversion of pyruvate to acetyl-CoA

8

Homomeric acetyl-CoA carboxylase

1

124

3711

Conversion of acetyl-CoA to malonyl-CoA

9

Heteromeric acetyl-CoA carboxylase biotin carboxylase subunit

1

1836

1972*

Conversion of acetyl-CoA to malonyl-CoA

10

Heteromeric acetyl-CoA carboxylase carboxyl transferase alpha subunit

1

2789

1609*

Conversion of acetyl-CoA to malonyl-CoA

11

Heteromeric acetyl-CoA carboxylase carboxyl transferase beta subunit

1

2079

1868*

Conversion of acetyl-CoA to malonyl-CoA

12

Heteromeric acetyl-CoA carboxylase biotin carboxyl carrier protein

2

3458, 4048

1388*,1232*

Conversion of acetyl-CoA to malonyl-CoA

13

Acyl carrier protein

2

13541,7450

437*,738*

Conversion of malonyl-CoA to malonyl-ACP

14

Beta-ketoacyl-ACP synthase III

1

4145

1204

Conversion of malony-ACP to 3-keto acyl ACP

15

3-Ketoacyl-ACP reductase

1

6732

1455*

Conversion of 3-keto acyl ACP to 3-hydroxy acyl ACP

16

Beta-ketoacyl-ACP synthase I

1

1954

1915*

Serial conversion of butyryl-ACP to palmitoyl-ACP

17

Beta-ketoacyl-ACP synthase II

1

11467

510

Conversion of palmitoyl ACP to Stearoyl-ACP

18

Stearoyl-ACP desaturase

4

6477,7609,2187, 11778

840, 727,1823*,496

Conversion of Stearoyl-ACP to oleoyl-ACP

19

Delta-12 fatty acid desaturase

2

1974, 3130

1914*, 1508*

Conversion of oleoyl-ACP to linoleoyl-ACP

20

Omega-3 fatty acid desaturase

2

1744,1847

2015*,1767*

Conversion of linoleoyl-ACP to α-linolenyl-ACP

21

Fatty acyl-ACP thioesterase B

1

4664

1098

Conversion of saturated fatty acyl-ACP to saturated free fatty acid

22

Palmitoyl-ACP thioesterase

1

7152

769

Hydrolysis of Palmitoyl-ACP to palmitic acid

23

Fatty acyl-ACP thioesterase A

1

5723

1452*

Conversion of unsaturated fatty acyl-ACP to unsaturated free fatty acid

24

Long chain acyl CoA synthetase

4

910,932,1027, 9748

2539*,2516*, 2442*,579

Activation free fatty acid to fatty acyl-CoA

25

Acyl-CoA binding protein

5

3898,955,6476, 13592, 10829

1267,2497*, 840, 436, 524*

Transport of acyl-CoA to the ER

26

Glycerol-3-phosphate acyl transferase

3

4004, 7597, 8581

1244,729,643

Acylation of glycerol-3-phosphate to lysophosphatidic acid

27

Lysophosphatidic acid acyl transferase

5

10599,6878, 11276,12982,6748

543, 799, 511, 462, 811

Acylation of lysophosphatidic acid to phosphatidic acid

28

Phosphatidic acid phosphatase

1

10375

549

Conversion of phosphatidic acid to diacyl glycerol

29

Diacyl glycerol acyl transferase

2

11532,14284

509,361

Acylation of diacyl glycerol to triacyl glycerol

Total number of genes

56