Interactions of a pesticide/heavy metal mixture in marine bivalves: a transcriptomic assessment

BMC Genomics

Table 4 Real time Q-PCR analysis: comparison with microarray data

				Ni			Chlorpyrifos			Mix
Array_ID	EMBL ID	Description	Q-PCR	M	B	Q-PCR	M	B	Q-PCR	M	B
Myt01-016C08	AJ625847	metallothionein isoform mt-10b	2.8*	1.46	11.5	-0.3	-0.1	-6.3	1.8*	0.9	4.4
N.A.	AY566247	metallothionein isoform mt-20-IV	0.6	N.A.	N.A.	-0.1	N.A.	N.A.	0.0	N.A.	N.A.
Myt01-009A12	AJ624405	gm2 ganglioside activator protein	13.6*	2.63	7.0	-0.7	-0.3	-5.4	-7.6*	0.5	-1.3
Myt01-009E10	AJ624495	gm2 ganglioside activator protein	-4.4*	-0.87	9.7	5.6*	-0.6	-5.0	6.7*	0.4	0.1
Myt01-016D06	AJ625863	apolipophorin precursor	0.8*	0.56	5.1	0.3	-0.1	-6.1	-0.6	-0.0	-6.4
Myt01-015B01	AJ625569	chitinase	1.7	-0.08	-7.2	6.4*	1.9	12.2	6.6*	1.7	4.7
Myt01-007F12	AJ624093	chitinase	1.5	-0.41	2.3	5.4*	1.9	9.9	5.6*	2.0	0.4
Myt01-010C02	AJ624637	chitinase	0.9	-0.52	3.6	5.7*	1.6	4.5	6.0*	1.7	1.8
Myt01-012D02	AJ625051	chitinase 1	0.4	-0.8	7.5	5.1*	3.2	11.5	6.2*	2.0	0.3
Myt01-004H06	AJ623463	beta-n-acetyl-hexosaminidase	-0.1	0.03	-7.5	-0.4	-0.3	-6.1	2.9*	1.1	-5.7
Myt01-012F08	AJ625116	actin	-0.0	-0.05	-6.9	0.4	0.1	-6.3	0.6*	0.5	2.7
Myt01-013C11	AJ625243	p-53 like	0.1	-0.11	-7.2	-0.4	0.3	-6.3	-0.1	-0.6	-3.9
Match (absolute; relative)				8; 0.73			10; 0.91			9; 0.82

Shown are: Array_ID, unique identifier; EMBL ID, EMBL database gene identifier; Description, B2GO annotation; Q-PCR, log2 mean relative expression level, (to non exposed reference mussels) obtained by means of Q-PCR analysis; M, relative expression level obtained by means of microarray analysis; B, Bayes Log Odd, where B > 0 denotes a statistically significant differentially expressed gene in microarray analysis.
Match indicates the absolute and relative frequency of accordance between microarray and Q-PCR analysis for each class of treatments. A match was assigned whether (i) a pairwise comparison showed the same statistically significant (positive or negative) expression trend or (ii) independently on the expression trend, when both analysis outcomes were not statistically significant. The AY566247 gene was not present in the microarray and therefore it was not considered for this analysis.
Relative expression levels were geometrically normalized against a 18 rRNA ribosomal target and an invariant microarray gene, alkaline phosphatase. * (p < 0.05; n = 4), threshold cycle reallocation randomization test according to [74]. N.A., not available

ISSN: 1471-2164