Figure 9
Pathway analysis of FBPA clustering on gene expression in the bystander response. FBPA clusters 2, 3 and 4 are shown here as networks generated by mapping genes using Agilent IDs and using the "grow" tool in IPA to identify potential transcriptional regulators upstream of genes in that cluster. Arrows indicate the direction of activity of a protein (empty node) interacting with the gene or gene product that is differentially regulated (colored node). Nodes are colored by log2 gene expression ratios in irradiated cells at 4 hours. On the scale bar, red indicates up-regulation and green, down-regulation of mRNA compared to sham-irradiated controls. Highlighted nodes indicate known transcriptional regulators involved in the radiation gene response in IMR-90 fibroblasts (violet outline) and potential new regulators of transcription (orange outline) suggested by the IPA analyses.