Figure 5
Organization of representative SSRs in extended DNA fibres from neuroblast nuclei of D. melanogaster after ND-FISH with the indicated probes (green or red signals from digoxigenin- and biotin-labelled probes respectively) and DAPI (blue) staining. An example of two colour fibre ND-FISH in haloed nuclei with (AAGAC)3 (red) and (AACAC)3 (green) is shown in a. Examples of fluorescent signal patterns in fibres stretched to different degrees are shown in e (square insets). Note the characteristic beaded nature of the signals in the highly extended fibres. A representative of the gaps observed between the continuous track of beaded signals is amplified in f. Scale bar = 10 μm, except in f in which it represents 5 μm. Every micrometer represents 3 kb of the highly stretched DNA.