Figure 3

Localization of NPAS4 in the brain of WT and Gpr54 -/- mice. For clarity the brain regions represented in panels A-F are shown mapped to an equivalent coronal section of the Allen brain atlas, corresponding to coronal level 77 (http://mouse.brain-map.org). The conceptual nuclear organization is shown in the right half of the panel, annotations are available at the brain atlas website. (A) WT mouse hippocampus stained with NPAS4 antibody (B) Gpr54 knockout (GKO) hippocampus stained with beta gal (blue cell bodies) and NPAS4 (red-brown). (C) WT cortex stained with NPAS4 antibody (red-brown) (D) GKO cortex stained with beta-gal (blue) for Gpr54 transcription and NPAS4 protein (red-brown) (E) WT posterior hypothalamus stained with NPAS4 antibody (red-brown) (F) GKO posterior hypothalamus stained with beta gal (blue) for Gpr54 transcription and NPAS4 protein (red-brown). (G) a more anterior coronal section (level 71, map not shown here) in GKO showing GPR54 transcription (blue, beta gal) and NPAS4 immunoreactivity in the periventricular hypothalamus. No endogenous beta-gal activity was seen in the wild-type mice in any of the brain regions. MT: mammilothalamic tract. 3 V: third ventricle.