Figure 1

Multiple sequence alignment of gene predictions for mycofactocin precursors. All detectable members of the family defined by TIGR03969 were collected, sorted by length, and aligned by MUSCLE [33], and then made non-redundant to 80% sequence identity, preferentially keeping sequences previously treated as genes and available through NCBI. Significant sequence similarity is restricted to the last 23 amino acids; the seven invariant residues occur among the last eight positions.